The Knockout Mouse Phenotyping Program (KOMP2) is producing and phenotyping 2,500 knockout mouse lines from gene targeted ES cells generated by the first phase of the Knockout Mouse Project (KOMP). Three consortia are collaborating to make this happen.
TCP is providing expertise and resources to several areas of activity within the DTCC:
KOMP2 involves production of mouse lines from ES cells as the first step. TCP Transgenic Core is using morula aggregation to produce germ line transmitting chimeras from KOMP ES cells for the DTCC project. Beyond the DTCC, TCP Transgenic Core also uses morula aggegration to produce mouse lines for the NorCOMM2 project and offers fee-for-service generation of chimeras from ES cells, production of transgenic mice by pronuclear injection, re-derivation of mice to SPF status, and ES cell targeting.
The DTCC project crypopreserves each mouse line for storage and distribution to the international research community. At TCP, the Canadian Mouse Mutant Repository (CMMR) will cryopreserve mutant mouse lines as sperm that is available on request from the KOMP repository. Beyond the DTCC, CMMR services include sperm and embryo cryopreservation and cryorecovery, and archive and storage of tissue and cell samples using state-of-the-art technologies on a cost-recovery basis.
Most of the baseline adult phenotyping for the DTCC project will be done at UCDavis. However, Clinical & Pathology Phenotyping Cores at TCP also has adult phenotyping capacity. Key phenotyping expertise that TCP brings to the DTCC is the ability to phenotype embryonic lethal lines using 3D technologies at the Mouse Imaging Centre (MICe) to identify key organ and/or tissue dysmorphologies contributing to embryonic death. The Clinical & Pathology Phenotyping Cores will also develop a challenge adult phenotyping screen to assess cardiac function (arrhythmia in response to drugs), respiratory function (lung function in response to methylcholine challenge) and stem cell function (repopulation of hematopoetic system after treatment with 5-fluorouracil). Beyond the DTCC, the Clinical & Pathology Phenotyping Cores provide all adult and embryonic lethal phenotyping for the NorCOMM2 project and offers in-life and pathology phenotyping services on a fee-for-service basis. A full list of phenotyping tests available can be found online.